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IONS抗體說明書

時間:2012-7-5閱讀:1708
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Product type:primary antibody               Host: rabbit               Clonality: polyclonal
 
Immunogen:Recombinant Protein            Storage: Long term: -20°C; Short term: +4°C                              
 
Tested Reacted Species: Human, Rat,mouse     Isotype: IgG               Mol wt: 130kDa
RecommendedApplication: WB,ELISA,IP,IHC-P,IHC-F                    Size:100ul/200ul(1mg/ml)
 
Synonyms: i NOS; Nitric Oxide Synthase, Inducible; HEP NOS; Hepatocyte NOS; HEPNOS; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; INOS; Inosl; MAC NOS;
 
Background InformationNitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
Key Applications
· WB=1:100-500    ELISA=1:500-1000   IP=1:20-100  IHC-P=1:100-500   IHC-F=1:100-500 
· Optimal working dilutions must be determined by the end user.
 
StorageStore at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01 M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ºC.
 
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 

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